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產(chǎn)品目錄
  • 細(xì)胞培養(yǎng)進(jìn)口血清
    進(jìn)口胎牛血清
    進(jìn)口新生牛血清
    進(jìn)口豬血清
    馬血清
  • 支原體檢測(cè)盒及標(biāo)準(zhǔn)品
    常規(guī)PCR檢測(cè)試劑盒
    熒光定量PCR檢測(cè)(qPCR法)
    支原體DNA提取
    靈敏度標(biāo)準(zhǔn)品(方法驗(yàn)證用)
    特異性標(biāo)準(zhǔn)品(方法驗(yàn)證用)
    PCR定量標(biāo)準(zhǔn)品(可用于方法驗(yàn)證)
  • 支原體祛除試劑
    細(xì)胞中支原體祛除
    環(huán)境支原體祛除
    水槽支原體祛除
  • 干細(xì)胞培養(yǎng)基
  • DNA/RNA污染祛除
    DNA/RNA污染祛除試劑
    DNA污染監(jiān)測(cè)
  • RNA病毒研究試劑
    RNA病毒檢測(cè)試劑盒
    病毒RNA提取
  • PCR儀器及配套產(chǎn)品
    DNA污染監(jiān)測(cè)祛除
    PCR/qPCR儀性能檢查
    PCR試劑
    PCR試劑盒
    PCR預(yù)混液(凍干粉)
    熱啟動(dòng)聚合酶MB Taq DNA
  • 微生物PCR檢測(cè)
    食品檢測(cè)類產(chǎn)品
    食品微生物檢測(cè)
    細(xì)菌PCR檢測(cè)

比較研究胎牛血清和馬血清對(duì)初級(jí)馬支氣管成纖維細(xì)胞的生長(zhǎng)和分化的影響

2016-09-23 13:25

Cell yield from digested bronchial tissue was consistent. Under both culture conditions, EBF were stained with trypan blue and the percentage of viable cells was similar, usually?>?95%. No significant evidence of cell necrosis or cell apoptosis was observed under inverted light microscopic analysis. With regard to cell morphology, EBF cultured in DMEM with 10% FBS appeared to be typically flattened and spindle-shaped (with a homogenous cytoplasm) over several weeks of passages compared to EBF cultured in DMEM with 10% HS (Figure 1A). In medium containing FBS, EBF were grown over the growth surface with only loose cell-cell-contact until reaching confluence and then formed tight parallel lines which remained as typical fibroblastic monolayer until passage 15. EBF between passages 16 – 20 started to change their morphology: cells were large, flat and more polygonal shaped, with a large, heterogeneous nucleus. At the same time, cell growth was rapidly reduced and cell viability was diminished. In contrast, EBF cultured in the presence of HS showed altered morphological changes within 2 days of culture; cells were small and more compact in shape combined with granula-like dark structures in the cytoplasm (Figure 1C). Moreover, cells grew in clusters and chains and failed to reach confluence within a week. This morphological behaviour of EBF was seen under this culture conditions until passage 7, but thereafter, EBF failed to proliferate regularly and decreased in their viability (passage 9). Under both culture conditions, most EBF (>99%) were positive for vimentin, but with more characteristic filamentous structures within the cytoplasm in medium containing FBS (Figure 1B) than in the presence of HS (Figure 1D).

In sum, serum addition to basal EBF medium enhanced EBF differentiation into myofibroblasts, and these findings are useful to develop in vitro fibroblast culture models that mimic in vivo physiological processes and to study airway disease mechanisms and remodeling.

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