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產(chǎn)品目錄
  • 細(xì)胞培養(yǎng)進(jìn)口血清
    進(jìn)口胎牛血清
    進(jìn)口新生牛血清
    進(jìn)口豬血清
    馬血清
  • 支原體檢測(cè)盒及標(biāo)準(zhǔn)品
    常規(guī)PCR檢測(cè)試劑盒
    熒光定量PCR檢測(cè)(qPCR法)
    支原體DNA提取
    靈敏度標(biāo)準(zhǔn)品(方法驗(yàn)證用)
    特異性標(biāo)準(zhǔn)品(方法驗(yàn)證用)
    PCR定量標(biāo)準(zhǔn)品(可用于方法驗(yàn)證)
  • 支原體祛除試劑
    細(xì)胞中支原體祛除
    環(huán)境支原體祛除
    水槽支原體祛除
  • 干細(xì)胞培養(yǎng)基
  • DNA/RNA污染祛除
    DNA/RNA污染祛除試劑
    DNA污染監(jiān)測(cè)
  • RNA病毒研究試劑
    RNA病毒檢測(cè)試劑盒
    病毒RNA提取
  • PCR儀器及配套產(chǎn)品
    DNA污染監(jiān)測(cè)祛除
    PCR/qPCR儀性能檢查
    PCR試劑
    PCR試劑盒
    PCR預(yù)混液(凍干粉)
    熱啟動(dòng)聚合酶MB Taq DNA
  • 微生物PCR檢測(cè)
    食品檢測(cè)類產(chǎn)品
    食品微生物檢測(cè)
    細(xì)菌PCR檢測(cè)

胎牛血清和血清蛋白組分對(duì)脂質(zhì)體的磷脂酰膽堿的攝取作用由大鼠肝細(xì)胞原代單層培養(yǎng)

2016-09-27 14:27

However, we found that the presence of fetal calf serum in the culture medium greatly diminished rather than increased the total transfer of liposomal lipid to the cells. Also bovine serum albumin and bovine β-globulins reduced this transfer, although to a lesser extent than whole serum. α-Globulins, on the other hand, were as effective as complete serum in reducing the uptake of liposomal phospholipid. A γ-globulin fraction failed to exhibit any effect on the uptake of [14C]phosphatidylcholine by the cells.

All protein fractions which were able to inhibit cellular uptake of liposomal phospholipid were shown to bind to the phospholipid vesicles. Furthermore, lipid vesicles preincubated with fetal calf serum and then separated from it showed reduced transfer of labeled phosphatidylcholine to parenchymal cells.

These observations were taken to suggest that the diminished uptake of liposomal lipid may be caused by a modification of the liposomal surface membrane as a result of the binding of certain serum proteins.

文章引自:ScienceDirect;版權(quán)聲明:版權(quán)歸原作者所有,如有版權(quán)問題,請(qǐng)與我們聯(lián)系。


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