微生物培養(yǎng)國際流行 — 非動(dòng)物源性共增菌培養(yǎng)基：產(chǎn)品詳細(xì)說明

產(chǎn)品1、植物源性TSB

（Soyabean HiVeg Medium ）

貨號(hào)：MV011

適合大腸桿菌、沙門氏菌、李斯特菌、金黃色葡萄球菌、肺炎鏈球菌、白色念珠菌、巴西曲霉菌、銅綠假單胞菌、枯草芽孢桿菌等大多數(shù)菌的生長繁殖。

Soyabean HiVeg Medium is a general purpose medium used for cultivation of a wide variety of microorganisms and recommended for sterility testing of moulds and lower bacteria.

大豆植物源性培養(yǎng)基是通用型培養(yǎng)基，用于廣泛培養(yǎng)各種微生物。也推薦用于霉菌和細(xì)菌的無菌檢查。

Composition**  （構(gòu)成成分）

Directions （說明）

Suspend 30 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Dispense as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 25°C.

1000ml水中加30g培養(yǎng)基，如果需要可加熱使其完全溶解，然后進(jìn)行分裝即可。高壓滅菌 （15磅121℃） 15分鐘。冷卻至25℃。

Note: If any fibres are observed in the solution, it is recommended to filter the solution through a 0.22 micron filter to eliminate the possibility of presence of fibres.

注：若溶液中有纖維，建議用0.22μm的濾膜過濾。

Principle And Interpretation （原理和說明）

Soyabean HiVeg Medium is prepared by completely replacing animal based peptones with vegetable peptones that makes the medium free of BSE/TSE risks. It is the modification of Soyabean Casein Digest Medium recommended by various pharmacopeias for sterility testing of various products and sensitivity testing of antimicrobial agents by tube dilution method (1-3) . This is a very nutritious medium supporting the growth of a variety of organisms (4). The combination of HiVeg hydrolysate and papaic digest of soyabean meal makes this medium nutritious by providing amino acids and long chain peptides for the growth of microorganisms. Dextrose and dipotassium phosphate serves as the carbohydrate source and the buffer in the medium. Sodium chloride maintains the osmotic balance of the medium.

此產(chǎn)品是植物源性蛋白胨配置而成，可完全取代動(dòng)物源性蛋白胨，從而避免瘋牛病的風(fēng)險(xiǎn)。它是各種藥典推薦進(jìn)行無菌檢查和藥敏實(shí)驗(yàn)用的胰蛋白胨大豆肉湯培養(yǎng)基的改良型。它是一種非常富有營養(yǎng)的培養(yǎng)基，支持各種微生物的生長。植物源蛋白水解物和大豆粉水解物的結(jié)合， 可提供微生物生長所必需的氨基酸和長鏈多肽。葡萄糖和K₂HPO₄分別提供碳源及緩沖，Nacl維持滲透壓平衡。

Quality Control（質(zhì)量控制）

Appearance（外觀）

Cream to yellow homogeneous free flowing powder

乳黃色均一自由流動(dòng)的粉末。

Colour and Clarity of prepared medium

Light yellow coloured clear solution without any precipitate.

淡黃色澄清溶液，無沉淀。

Reaction

pH of 3.00% w/v aqueous solution at 25°C (after sterilization). pH : 7.3±0.2

在25℃且3%的水溶液條件下，滅菌后其PH值為7.3±0.2。

pH

7.10-7.50

Stability test 穩(wěn)定性

Light yellow coloured clear solution without any precipitation or sedimentation at room temperature for 7 days

室溫存放7天，仍呈現(xiàn)淡黃色、澄清、無沉淀的溶液。

Growth promoting properties （生長支持性）

Clearly visible growth of microorganism comparable to that previously obtained with previously tested and approved lot of medium occurs at the specified temperature for not more than the shortest period of time specified inoculating <=100 cfu(at 30-35°C for 18-24 hours for bacteria and 5 days for fungal). Growth promotion is carried out as per USP/EP/BP/JP.

清晰可見的微生物的生長與先前測試和批準(zhǔn)的培養(yǎng)基批號(hào)所獲得的微生物相當(dāng)。培養(yǎng)條件為接種≤100cfu，在30-35℃下細(xì)菌培養(yǎng)18-24小時(shí)，真菌培養(yǎng)5天。生長促進(jìn)流程按《美國藥典》、《歐洲藥典》、《英國藥典》、《日本藥典》進(jìn)行。

Sterility Testing + Validation （無菌檢查驗(yàn)證）

The medium is tested with suitable strains of microrganisms inoculating <=100cfu and incubating at 20-25°C for not more than 3 days in case of bacteria and not more than 5 days in case of fungi.

培養(yǎng)基采用接種量≤100cfu的適當(dāng)?shù)奈⑸锞赀M(jìn)行檢查。細(xì)菌在20-25°C孵育不超過3天，真菌不超過5天。

Cultural Response （培養(yǎng)反應(yīng)）

Storage and Shelf Life （儲(chǔ)存與保質(zhì)期）

Store below 30°C in tightly closed container and prepared medium at 2-8°C. Use before expiry date on label.

30°C以下密閉保存，制備好的培養(yǎng)基在2-8°C保存。標(biāo)簽上的截止日期前使用。

Reference （參考文獻(xiàn)）

1.MacFaddin, J. F. 1985. Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria vol. 1. Baltimore: Williams and Wilkins.

2.The United States Pharmacopeia, 2008, USP31/NF26, The United States Pharmacopeial Convention, Rockville, MD. 3.Indian Pharmacopeia, 2007, Govt. of India, Ministry of Health and Family Welfare, New Delhi, India.

4.Forbes, B. A., Sahm, D. F . and Weissfield, A. S. 2002. Bailey and Scott's Diagnostic Microbiology. 11 ed. St Louis: The C.V. Mosby Co.

Revision : 1 / 2011

Disclaimer :

User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained in this and other related HiMedia? publications. The information contained in this publication is based on our research and development work and is to the best of our knowledge true and accurate. HiMedia? Laboratories Pvt Ltd reserves the right to make changes to specifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use but for laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not be considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any patents.

附表1：植物源性TSB參考報(bào)價(jià)

產(chǎn)品2、植物源性腦心浸出液肉湯 （植物源性BHI）

Brain Heart Infusion Broth, HiVeg/ Brain Heart              

貨號(hào)：MV210/MV1036/MV1037

適合對(duì)營養(yǎng)要求不高的普通菌種以及對(duì)營養(yǎng)挑剔的腦膜炎奈瑟菌、肺炎鏈球菌、釀膿鏈球菌、金黃色葡萄球菌等菌種的生長繁殖。

Infusion, with 0.1% Agar, HiVeg^TM / with 6.5% NaCl, HiVeg^TM

Brain Heart Infusion Broth, HiVeg / Brain Heart Infusion, with 0.1% Agar, HiVeg / with 6.5% NaCl, HiVeg is employed for the propagation of fastidious pathogenic cocci and other organisms associated with blood culture work and allied pathological investigations.

植物源性腦心浸出液肉湯 / 含0.1%瓊脂的植物源性腦心浸出液肉湯/含6.5% NaCl的植物源性腦心浸出液肉湯主要用于繁殖對(duì)營養(yǎng)挑剔的病原球菌和其他與血培養(yǎng)相關(guān)的微生物及聯(lián)合病理檢查。

Final pH (at 25°C) 7.4 ± 0.2（最終pH值 (25°C) 7.4 ± 0.2）

** Formula adjusted, standardized to suit performance parameters

Directions :

Suspend 37.0 grams of MV210 or 38.0 grams of MV1036 or 97.0 grams of MV1037 in 1000 ml distilled water. Dispense into bottles or tubes and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. For best results, the medium should be used on the day it is prepared, otherwise, it should be boiled or steamed for a few minutes and then cooled before use.

在1000ml蒸餾水中重懸37.0克MV210或38.0克MV1036或97.0克MV1037。用三角燒瓶或試管分裝，并15磅壓力（121℃）下高壓滅菌15分鐘。為了保證**效果，**配制當(dāng)天就使用，否則需要蒸/煮幾分鐘，冷卻后方可使用。

Principle and Interpretation (原理和說明)：

These media are prepared by completely replacing animal based peptone with vegetable peptone making the media free of BSE / TSE risks. Rosenow (1) devised the original medium by adding brain tissue to dextrose broth. These media like the conventional media are nutritious and well buffered to support the growth of wide variety of microorganisms (2, 3, 4). With the addition of 10% defibrinated sheep blood, it is useful for isolation and cultivation of Histoplasma capsulatum (5) and other fungi. In the formulation containing 6.5% sodium chloride (MV1037), the salt acts as a differential and/or selective agent by interfering with membrane permeability and osmotic and electrokinetic equilibria in salt intolerant organisms. The addition of 0.1% agar improves growth of microaerophilic and anaerobic microorganisms (4). Brain Heart Infusion Broth, HiVeg with addition of 1.5% agar should not be used for detection of haemolytic activity of Streptococci, since it contains dextrose, which has been reported to cause a typical haemolytic reactions when it is present in blood containing media. For selective isolation of fungi, addition of Gentamicin and/or Chloramphenicol is recommended (6).

這些培養(yǎng)基通過用植物蛋白胨完全替代動(dòng)物源蛋白胨而制備，從而不具有瘋牛病風(fēng)險(xiǎn)。 Rosenow通過將腦組織添加到葡萄糖肉湯中設(shè)計(jì)了最初的培養(yǎng)基。這些培養(yǎng)基和傳統(tǒng)培養(yǎng)基一樣富有營養(yǎng)和良好的緩沖能力，支持各種微生物的生長。加入10％去纖維綿羊血后，可用于莢膜組織胞漿菌等真菌的分離培養(yǎng)。在含有6.5％氯化鈉（MV1037）的配方中，該鹽干擾不耐鹽微生物的膜滲透性和滲透壓來作為差異性選擇因子。添加0.1％的瓊脂（MV1036）可改善微量需氧菌和厭氧菌的生長。添加1.5％瓊脂的HiVeg腦心浸出液肉湯不應(yīng)用于檢測鏈球菌的溶血活性，因?yàn)樗械钠咸烟窃诤囵B(yǎng)基中據(jù)報(bào)道會(huì)引起典型的溶血反應(yīng)。對(duì)于選擇性分離真菌，建議添加慶大霉素和/或氯霉素。

Quality Control :

Appearance of Powder

Yellow coloured may have slightly greenish tinge, homogeneous, free flowing powder.

Colour and Clarity

Light amber coloured, clear to slightly opalescent solution.

Reaction

Reaction of 3.7% w/v of MV210, 3.8%w/v of MV1036 or 9.7%w/v of MV1037 aqueous solution is pH 7.4 ± 0.2 at 25°C.

Cultural Response

Cultural characteristics observed after an incubation at 35-37°C for 24-48 hours.

Key : *= on MV210, MV1036

**= on MV1037

MV210Brain Heart Infusion Broth, HiVeg

1.Control

2.Neisseria meningitidis

3.Streptococcus pneumoniae

4.Streptococcus pyogenes

5.Staphylococcus aureuss

References:

1.Rosenow, 1919, J. Dental Research, 1:205.

2.Roseburg T. et al, 1944, J. Inf. Dis., 74:131.

3.Conant N.F., 1950, Diagnostic Procedures and Reagents, 3rd  ed., A.P.H.A. Inc.,New York.

4.MacFaddin  J.F.,  1985,  Media  for  Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. I, Williams and Wilkins, Baltimore.

5.Howard B., Keiser J.F., Weissfeld A., et al, 1994, Clinical and Pathogenic Microbiology, 2nd ed., Mosby Co.

6.Murray PR., Baron, Pfaller, Tenover and Yolken (Eds.), ASM, W^{ashington, D.C.}2003, In Manual of clinical Microbiology, 8th ed.

附表2：植物源性腦心浸出液肉湯（植物源性BHI）  參考報(bào)價(jià)

原創(chuàng)聲明：本文系威正翔禹生物原創(chuàng)，轉(zhuǎn)載請(qǐng)注明出處，違者必究！